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Can exonuclease be used while producing a recombinant DNA molecule?

Posted: Wed Nov 08, 2023 3:21 am
by tonylee
Can exonuclease be used while producing a recombinant DNA molecule?

Re: Can exonuclease be used while producing a recombinant DNA molecule?

Posted: Wed Nov 08, 2023 3:22 am
by suhasdas
No, an exonuclease cannot be used while producing a recombinant DNA. This is because an exonuclease degrades the DNA. It cannot produce DNA fragments with sticky ends.